Selecting an HPLC Column Supplier

When selecting an HPLC column supplier, you should carefully consider a number of factors. While price and company reputation are still the top two considerations, they are not necessarily the only ones. In addition to these, you should also consider the lifetime of the column. We'll discuss these issues in more detail in the next section.

The lifespan of an HPLC column is largely dependent on how well it performs. You should read the specification sheet carefully, and only use the column according to its specifications. Occasionally, you will use the column outside of its specifications, so be sure to carefully monitor its function and make necessary adjustments. For example, we once used a TSKgel SuperSW mAb-HTP column at a flow rate of 0.75 mL/min, which was higher than the recommended flow rate of 0.5 mL/min.

When it comes to HPLC column suppliers, users have two main choices: general laboratory distributors and chromatography supply houses. Compared to general laboratory distributors, chromatography supply houses enjoy higher market share. Although general laboratory suppliers still remain popular, their share continues to decline. Only 18% of users purchase their columns from general laboratory distributors. In addition, supply houses provide better technical support than general laboratory suppliers. In addition, these Chrom Tech suppliers tend to offer one-stop-shop services.

Another consideration is column efficiency. This refers to how well the column can resolve the peak. Higher resolution requires a longer column. In general, a resolution of 1.5 or above is acceptable. Therefore, the correct column length should be determined according to your separation goals. For example, a 15-cm column operating at 11 Mpa backpressure is sufficient for most purposes.

When selecting an HPLC column supplier, you should also consider the performance of the column. Certain additives have adverse effects on column performance, so you must make sure that you select the column that is compatible with the additives you intend to use. For example, if you're going to use a column to analyze proteins, you should choose a column made of a high-quality polymer.View this link chromtech.com for fast and consistent results for HPLC applications.

Particle size is also a consideration. Higher particle size reduces backpressure. It also increases selectivity. Smaller particle size increases the backpressure. In fact, pressure inversely proportional to particle size, so a smaller particle size increases the backpressure. Similarly, smaller particles minimize the interstitial voids and reduce the push-through of compounds.

Overall, column lifetime is increasing. However, users still need to purchase seven new columns each year. This may be related to increasing sample volumes or procurement of improved columns. In addition, the life of a column depends on how often it is used. The lifespan of a column reflects how many sample injections the column is subjected to. The longer a column lasts, the higher the overall efficiency of the HPLC instrument will be. Check out this post for more details related to this article: https://en.wikipedia.org/wiki/High-performance_liquid_chromatography.